Sant Pau - Institut de Recerca

Main Lines of Research

Development of novel animal models of disseminated disease in solid tumours and haematological neoplasms for the mechanistic study of metastases and preclinical drug development..
Identification of prognostic biomarkers in head and neck squamous cell carcinoma and their functional validation in vivo.
Development of nanoconjugates for targeted delivery of antitumour drugs or toxins to metastasis stem cells.

Challenges

Obtain funding to ensure stable employment of senior researchers, attract postdoctoral fellows and reinforce current collaborations with clinicians.
Develop molecular markers for therapeutic decision-making in head and neck carcinomas.
Achieve antimetastatic effects by targeting antitumour drugs to metastasis stem cells in solid tumors and haematological neoplàsies.
Enhance international collaboration and participation in EU networks and industrial projects.

Group coordination

MANGUES BAFALLUY, RAMON IR
rmangues@santpau.cat

Members

Alamo Vargas, Patricia Virginia ENTITAT EXTERNA
Alba Castellon, Lorena IR
Arena, Lourdes Alien IR
Carrasco Diaz, Luis Miguel IR
Casanova Riga, Isolda ENTITAT EXTERNA
Diez Serrano, Alvaro IR
Garcia Leon, Annabel CIBERBBN
Mendoza Fernandez, Julian Ignacio CIBERBBN
Miranda Tovar, Eva IR
Navas Jimenez, Luis Carlos IR
Romero Perez, Ines IR
Rueda Matas, Ariana IR
Seira Oriach, Clara IR
Sierra Cruz, Marta IR
Unzueta Elorza, Ugutz IR
Varona Alvarez, Saray IR

Publications 2023

(JIF 2022)

-Álamo P, Parladé E, Favaro MTP, Gallardo A, Mendoza R, Ferreira LCS, Roher N, Mangues R, Villaverde A, Vázquez E. Probing the Biosafety of Implantable Artificial Secretory Granules for the Sustained Release of Bioactive Proteins. ACS Applied Materials & Interfaces. 2023; 15(33)DOI:10.1021/acsami.3c08643. PMID:37614001. IF:9,500 (Q1/2D). Document type: Article.

-López H, Rueda A, Martínez C, Sánchez L, Carratalá JV, Atienza J, Parladé E, Sánchez JM, Serna N, Voltà E, Ferrer N, Reverter D, Mangues R, Villaverde A, Vázquez E, Unzueta U. Biofabrication of Self-Assembling Covalent Protein Nanoparticles through Histidine-Templated Cysteine Coupling. ACS Sustainable Chemistry & Engineering. 2023; 11(10)DOI:10.1021/acssuschemeng.2c06635. IF:8,400 (Q1/1D). Document type: Article.

-Martinez C, Alba L, Carrasco LI, Serna N, Imedio L, Gallardo A, Casanova I, Unzueta U, Vazquez E, Mangues R, Villaverde A. Lymphocyte infiltration and antitumoral effect promoted by cytotoxic inflammatory proteins formulated as self-assembling, protein-only nanoparticles. BIOMEDICINE & PHARMACOTHERAPY. 2023; 164:114976. DOI:10.1016/j.biopha.2023.114976. PMID:37276641. IF:7,500 (Q1/1D). Document type: Article.

-Medina E, García A, Gallardo A, Álamo P, Alba L, Unzueta U, Villaverde A, Vázquez E, Casanova I, Mangues R. Potent Anticancer Activity of CXCR4-Targeted Nanostructured Toxins in Aggressive Endometrial Cancer Models. Cancers. 2023; 15(1):85. DOI:10.3390/cancers15010085. PMID:36612081. IF:5,200 (Q2/3D). Document type: Article.

-Nunez Y, Garcia A, Falgas A, Serna N, Sanchez L, Garrido A, Sierra J, Gallardo A, Unzueta U, Vazquez E, Villaverde A, Mangues R, Casanova I. T22-PE24-H6 Nanotoxin Selectively Kills CXCR4-High Expressing AML Patient Cells In Vitro and Potently Blocks Dissemination In Vivo. Pharmaceutics. 2023; 15(3):727. DOI:10.3390/pharmaceutics15030727. PMID:36986589. IF:5,400 (Q1/2D). Document type: Article.

-Parladé E, Sánchez JM, López H, Unzueta U, Villaverde A, Vázquez E. Protein features instruct the secretion dynamics from metal-supported synthetic amyloids. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. 2023; 250:126164. DOI:10.1016/j.ijbiomac.2023.126164. PMID:37549767. IF:8,200 (Q1/1D). Document type: Article.

-Rueda A, Mendoza JI, Alba L, Parladé E, Voltà E, Paez D, Aviño A, Eritja R, Vázquez E, Villaverde A, Mangues R, Unzueta U. Site-directed cysteine coupling of disulfide-containing non-antibody carrier proteins (THIOCAPs). Science China-Materials. 2023; 66(10)DOI:10.1007/s40843-023-2571-6. IF:8,100 (Q1/3D). Document type: Article.

– Serna N, López H, Aceituno P, Rojas M, Parladé E, Voltà E, Martínez C, Sánchez JM, Di A, Carratalá JV, Livieri AL, Ferrer N, Vázquez E, Unzueta U, Roher N, Villaverde A. Efficient Delivery of Antimicrobial Peptides in an Innovative, Slow-Release Pharmacological Formulation. Pharmaceutics. 2023; 15(11):2632. DOI:10.3390/pharmaceutics15112632. PMID:38004610. IF:5,400 (Q1/2D). Document type: Article.

-Voltà E, Alba L, Serna N, Casanova I, López H, Gallardo A, Sánchez A, Villaverde A, Unzueta U, Vázquez E, Mangues R. High-precision targeting and destruction of cancer-associated PDGFR-ß(+) stromal fibroblasts through self-assembling, protein-only nanoparticles. Acta Biomaterialia. 2023; 170DOI:10.1016/j.actbio.2023.09.001. PMID:37683965. IF:9,700 (Q1/1D). Document type: Article.

-Voltà E, Parladé E, Serna N, Villaverde A, Vazquez E, Unzueta U. Endosomal escape for cell-targeted proteins. Going out after going in. BIOTECHNOLOGY ADVANCES. 2023; 63:108103. DOI:10.1016/j.biotechadv.2023.108103. PMID:36702197. IF:16,000 (Q1/1D). Document type: Review.

Publications 2022

(JIF 2022)

Barguilla I, Unzueta U, Carratala JV, Cano-Garrido O, Villaverde A, Hernandez A, Ferrer-Miralles N. Toxicity Profiling of Bacterial Inclusion Bodies in Human Caco-2 Cells. Frontiers in Bioengineering and Biotechnology. 2022; 10:842256. DOI:10.3389/fbioe.2022.842256. PMID:35573225. IF:5,7 (Q1/3D). Document type: Article.
Cano-Garrido O, Serna N, Unzueta U, Parlad E, Mangues R, Villaverde A, Vazquez E. Protein scaffolds in human clinics. BIOTECHNOLOGY ADVANCES. 2022; 61:108032. DOI:10.1016/j.biotechadv.2022.108032. PMID:36089254. IF:16 (Q1/1D). Document type: Review.
Falgàs A, Garcia-Leon A, Núñez Y, Serna N, Sánchez L, Unzueta U, Voltà E, Aragó M, Álamo P, Alba-Castellón L, Sierra J, Gallardo A, Villaverde A, Vázquez E, Mangues R, Casanova I. A diphtheria toxin-based nanoparticle achieves specific cytotoxic effect on CXCR4(+) lymphoma cells without toxicity in immunocompromised and immunocompetent mice. BIOMEDICINE & PHARMACOTHERAPY. 2022; 150:112940. DOI:10.1016/j.biopha.2022.112940. PMID:35421785. IF:7,5 (Q1/1D). Document type: Article.
López H, Voltà E, Parladé E, Villaverde A, Vázquez E, Unzueta U. Insights on the emerging biotechnology of histidine-rich peptides. BIOTECHNOLOGY ADVANCES. 2022; 54:107817. DOI:10.1016/j.biotechadv.2021.107817. PMID:34418503. IF:16 (Q1/1D). Document type: Review.
Medina E, Cespedes MV, Gallardo A, Rioja-Blanco E, Pavon MA, Asensio L, Farre L, Alba-Castellón L, Unzueta U, Villaverde A, Vazquez E, Casanova I, Mangues R. Novel Endometrial Cancer Models Using Sensitive Metastasis Tracing for CXCR4-Targeted Therapy in Advanced Disease. Biomedicines. 2022; 10(7):1680. DOI:10.3390/biomedicines10071680. PMID:35884987. IF:4,7 (Q1/3D). Document type: Article.
Pallares V, Unzueta U, Falgas A, Avino A, Nunez Y, Garcia-Leon A, Sánchez-García L, Serna N, Gallardo A, Alba-Castellón L, Alamo P, Sierra J, Cedo L, Eritja R, Villaverde A, Vazquez E, Casanova I, Mangues R. A multivalent Ara-C-prodrug nanoconjugate achieves selective ablation of leukemic cells in an acute myeloid leukemia mouse model. BIOMATERIALS. 2022; 280:121258. DOI:10.1016/j.biomaterials.2021.121258. PMID:34847435. IF:14 (Q1/1D). Document type: Article.
Parlade E, Volta-Duran E, Cano-Garrido O, Sanchez JM, Unzueta U, López-Laguna H, Serna N, Cano M, Rodríguez-Mariscal M, Vazquez E, Villaverde A. An In Silico Methodology That Facilitates Decision Making in the Engineering of Nanoscale Protein Materials. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022; 23(9):4958. DOI:10.3390/ijms23094958. PMID:35563346. IF:5,6 (Q1/3D). Document type: Article.
Parra AM, Perez A, Gris I, Bonel GC, Carrasco LM, Mokhtari K, Garcia L, Lupianez JA, Rufino EE. Involvement of the PI3K/AKT Intracellular Signaling Pathway in the AntiCancer Activity of Hydroxytyrosol, a Polyphenol from Olea europaea, in Hematological Cells and Implication of HSP60 Levels in Its Anti-Inflammatory Activity. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022; 23(13):7053. DOI:10.3390/ijms23137053. PMID:35806065. IF:5,6 (Q1/3D). Document type: Article.
Rioja-Blanco E, Arroyo I, Alamo P, Casanova I, Gallardo A, Unzueta U, Serna N, Sánchez-García L, Quer M, Villaverde A, Vazquez E, Leon X, Alba-Castellón L, Mangues R. CXCR4-targeted nanotoxins induce GSDME-dependent pyroptosis in head and neck squamous cell carcinoma. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH. 2022; 41(1):49. DOI:10.1186/s13046-022-02267-8. PMID:35120582. IF:11,3 (Q1/1D). Document type: Article.
Rioja-Blanco E, Arroyo I, Álamo P, Casanova I, Gallardo A, Unzueta U, Serna N, Sánchez-García L, Quer M, Villaverde A, Vázquez E, Mangues R, Alba-Castellón L, León X. Self-assembling protein nanocarrier for selective delivery of cytotoxic polypeptides to CXCR4(+) head and neck squamous cell carcinoma tumors. Acta Pharmaceutica Sinica B. 2022; 12(5). DOI:10.1016/j.apsb.2021.09.030. PMID:35646535. IF:14,5 (Q1/1D). Document type: Article.
Rioja-Blanco E, Gallardo A, Arroyo I, Alamo P, Casanova I, Unzueta U, Serna N, Sánchez-García L, Quer M, Villaverde A, Vazquez E, Leon X, Alba-Castellón L, Mangues R. A Novel CXCR4-Targeted Diphtheria Toxin Nanoparticle Inhibits Invasion and Metastatic Dissemination in a Head and Neck Squamous Cell Carcinoma Mouse Model. Pharmaceutics. 2022; 14(4):887. DOI:10.3390/pharmaceutics14040887. PMID:35456719. IF:5,4 (Q1/2D). Document type: Article.
Sala R, Rioja-Blanco E, Serna N, Sánchez-García L, Alamo P, Alba-Castellón L, Casanova I, Lopez A, Unzueta U, Cespedes MV, Vazquez E, Villaverde A, Mangues R. GSDMD-dependent pyroptotic induction by a multivalent CXCR4-targeted nanotoxin blocks colorectal cancer metastases. DRUG DELIVERY. 2022; 29(1). DOI:10.1080/10717544.2022.2069302. PMID:35532120. IF:6 (Q1/2D). Document type: Article.
Sanchez JM, Carratala JV, Serna N, Unzueta U, Nolan V, Sánchez-Chardi A, Volta-Duran E, López-Laguna H, Ferrer-Miralles N, Villaverde A, Vazquez E. The Poly-Histidine Tag H6 Mediates Structural and Functional Properties of Disintegrating, Protein-Releasing Inclusion Bodies. Pharmaceutics. 2022; 14(3):602. DOI:10.3390/pharmaceutics14030602. PMID:35335976. IF:5,4 (Q1/2D). Document type: Article.
Serna N, Falgas A, Garcia-Leon A, Unzueta U, Nunez Y, Sánchez-Chardi A, Martínez-Torró C, Mangues R, Vazquez E, Casanova I, Villaverde A. Time-Prolonged Release of Tumor-Targeted Protein-MMAE Nanoconjugates from Implantable Hybrid Materials. 2022; 14(1):192. DOI:10.3390/pharmaceutics14010192. PMID:35057088. IF:5,4 (Q1/2D). Document type: Article.
Serna N, Pallares V, Unzueta U, Garcia-Leon A, Volta-Duran E, Sánchez-Chardi A, Parlade E, Rueda A, Casanova I, Falgas A, Alba-Castellón L, Sierra J, Villaverde A, Vazquez E, Mangues R. Engineering non-antibody human proteins as efficient scaffolds for selective, receptor-targeted drug delivery. JOURNAL OF CONTROLLED RELEASE. 2022; 343. DOI:10.1016/j.jconrel.2022.01.017. PMID:35051493. IF:10,8 (Q1/1D). Document type: Article.
Unzueta U, Sanchez JM, Garcia-Fruitos E, Seras J. Editorial: High added-value nanoparticles: Rethinking and recycling cell protein waste. Frontiers in Bioengineering and Biotechnology. 2022; 10:1018014. DOI:10.3389/fbioe.2022.1018014. PMID:36131721. IF:5,7 (Q1/3D). Document type: Editorial material.
Volta-Duran E, Sanchez JM, H, Parlade E, Sánchez-García L, Sánchez-Chardi A, de Marco A, Unzueta U, Vazquez E, Villaverde A. The spectrum of building block conformers sustains the biophysical properties of clinically-oriented self-assembling protein nanoparticles. Science China-Materials. 2022; 65(6). DOI:10.1007/s40843-021-1914-0. IF:8,1 (Q1/2D). Document type: Article.
Volta-Duran E, Sanchez JM, Parlade E, Serna N, Vazquez E, Unzueta U, Villaverde A. The Diphtheria Toxin Translocation Domain Impairs Receptor Selectivity in Cancer Cell-Targeted Protein Nanoparticles. 2022; 14(12):2644. DOI:10.3390/pharmaceutics14122644. PMID:36559138. IF:5,4 (Q1/2D). Document type: Article.

Publications 2021

Alamo P, Cedano J, Conchillo O, Cano O, Alba L, Serna N, Avino A, Carrasco LM, Sanchez A, Martinez C, Gallardo A, Cano M, Eritja R, Villaverde A, Mangues R, Vazquez E, Unzueta U. Rational engineering of a human GFP-like protein scaffold for humanized targeted nanomedicines. Acta Biomaterialia. 2021; 130. DOI:10.1016/j.actbio.2021.06.001. PMID:34116228. IF:10,633.
Alamo P, Parlade E, Lopez H, Volta E, Unzueta U, Vazquez E, Mangues R, Villaverde A. Ion-dependent slow protein release from in vivo disintegrating micro-granules. DRUG DELIVERY. 2021; 28(1). DOI:10.1080/10717544.2021.1998249. PMID:34747685. IF:6,819.
Cano O, Alamo P, Sanchez L, Falgas A, Sanchez A, Serna N, Parlade E, Unzueta U, Roldan M, Volta E, Casanova I, Villaverde A, Mangues R, Vazquez E. Biparatopic Protein Nanoparticles for the Precision Therapy of CXCR4(+) Cancers. Cancers. 2021; 13(12):2929. DOI:10.3390/cancers13122929. PMID:34208189. IF:6,575.
Falgas A, Pallares V, Unzueta U, Nunez Y, Sierra J, Gallardo A, Alba L, Mangues MA, Alamo P, Villaverde A, Vazquez E, Mangues R, Casanova I. Specific Cytotoxic Effect of an Auristatin Nanoconjugate Towards CXCR4(+) Diffuse Large B-Cell Lymphoma Cells. International Journal of Nanomedicine. 2021; 16DOI:10.2147/IJN.S289733. PMID:33716502. IF:7,033.
Lopez H, Parlade E, Alamo P, Sanchez JM, Volta E, Serna N, Sanchez L, Cano O, Sanchez A, Villaverde A, Mangues R, Unzueta U, Vazquez E. In Vitro Fabrication of Microscale Secretory Granules. ADVANCED FUNCTIONAL MATERIALS. 2021; 31(21):2100914. DOI:10.1002/adfm.202100914. IF:19,924.
Lopez H, Sanchez JM, Carratala JV, Rojas M, Sanchez L, Parlade E, Sanchez A, Volta E, Serna N, Cano O, Flores S, Ferrer N, Nolan V, de Marco A, Roher N, Unzueta U, Vazquez E, Villaverde A. Biofabrication of functional protein nanoparticles through simple His-tag engineering. ACS Sustainable Chemistry & Engineering. 2021; 9(36). DOI:10.1021/acssuschemeng.1c04256. PMID:34603855. IF:9,224.
Pallares V, Nunez Y, Sanchez L, Falgas A, Serna N, Unzueta U, Gallardo A, Alba L, Alamo P, Sierra J, Villaverde A, Vazquez E, Casanova I, Mangues R. Antineoplastic effect of a diphtheria toxin-based nanoparticle targeting acute myeloid leukemia cells overexpressing CXCR4. JOURNAL OF CONTROLLED RELEASE. 2021; 335. DOI:10.1016/j.jconrel.2021.05.014. PMID:34004204. IF:11,467.
Sanchez JM, Lopez H, Serna N, Unzueta U, Clop PD, Villaverde A, Vazquez E. Engineering the Performance of Artificial Inclusion Bodies Built of Catalytic beta-Galactosidase. ACS Sustainable Chemistry & Engineering. 2021; 9(6). DOI:10.1021/acssuschemeng.0c08345. IF:9,224.
Sanchez L, Volta E, Parlade E, Mazzega E, Sanchez A, Serna N, Lopez H, Mitstorfer M, Unzueta U, Vazquez E, Villaverde A, de Marco A. Self-Assembled Nanobodies as Selectively Targeted, Nanostructured, and Multivalent Materials. ACS Applied Materials & Interfaces. 2021; 13(25). DOI:10.1021/acsami.1c08092. PMID:34129336. IF:10,383.
Serna N, Carratala JV, Conchillo O, Martinez C, Unzueta U, Mangues R, Ferrer N, Daura X, Vazquez E, Villaverde A. Antibacterial Activity of T22, a Specific Peptidic Ligand of the Tumoral Marker CXCR4. Pharmaceutics. 2021; 13(11):1922. DOI:10.3390/pharmaceutics13111922. PMID:34834337. IF:6,525.
Volta E, Serna N, Sanchez L, Avino A, Sanchez JM, Lopez H, Cano O, Casanova I, Mangues R, Eritja R, Vazquez E, Villaverde A, Unzueta U. Design and engineering of tumor-targeted, dual-acting cytotoxic nanoparticles. Acta Biomaterialia. 2021; 119. DOI:10.1016/j.actbio.2020.11.018. PMID:33189955. IF:10,633.

2017 Scientific Report -Oncogenesis and Antitumour

‘Agència de Gestió d’Ajuts Universitaris i de Recerca’ (AGAUR) funding:

DEVELOPMENT OF AN ANTIMETHTATIC NANOCONJUGATE DIRECTED TO CXCR4+ METASTATIC STEM CELLS (2014 PROD 00055)

Promote technological development, innovation and quality research

This project is cofunded by European Regional Development Fund (ERDF) allocated by the European Union in the frame of the catalan operative Programme 2014-2020 with 49,750.00€

U18. Nanotoxicology Unit (NANBIOSIS):

These equipment and services constitute unit 18 of NANBIOSIS, the integrated infrastructure for the production and characterization of nanomaterials, biomaterials and systems in biomedicine, of CIBER-BBN and the Minimal Invasion Surgery Center Jesús Usón, which has been recognized by Spanish Government as Unique Scientific-Technological Infrastructure (ICTS).

Introduction

Main Lines of Research

Development of novel animal models of disseminated disease in solid tumours and haematological neoplasms for the mechanistic study of metastases and preclinical drug development..
Identification of prognostic biomarkers in head and neck squamous cell carcinoma and their functional validation in vivo.
Development of nanoconjugates for targeted delivery of antitumour drugs or toxins to metastasis stem cells.

Challenges

Obtain funding to ensure stable employment of senior researchers, attract postdoctoral fellows and reinforce current collaborations with clinicians.
Develop molecular markers for therapeutic decision-making in head and neck carcinomas.
Achieve antimetastatic effects by targeting antitumour drugs to metastasis stem cells in solid tumors and haematological neoplàsies.
Enhance international collaboration and participation in EU networks and industrial projects.

Team

Group coordination

MANGUES BAFALLUY, RAMON IR
rmangues@santpau.cat

Members

Scientific output

Publications 2023

(JIF 2022)

Publications 2022

(JIF 2022)

Barguilla I, Unzueta U, Carratala JV, Cano-Garrido O, Villaverde A, Hernandez A, Ferrer-Miralles N. Toxicity Profiling of Bacterial Inclusion Bodies in Human Caco-2 Cells. Frontiers in Bioengineering and Biotechnology. 2022; 10:842256. DOI:10.3389/fbioe.2022.842256. PMID:35573225. IF:5,7 (Q1/3D). Document type: Article.
Cano-Garrido O, Serna N, Unzueta U, Parlad E, Mangues R, Villaverde A, Vazquez E. Protein scaffolds in human clinics. BIOTECHNOLOGY ADVANCES. 2022; 61:108032. DOI:10.1016/j.biotechadv.2022.108032. PMID:36089254. IF:16 (Q1/1D). Document type: Review.
Falgàs A, Garcia-Leon A, Núñez Y, Serna N, Sánchez L, Unzueta U, Voltà E, Aragó M, Álamo P, Alba-Castellón L, Sierra J, Gallardo A, Villaverde A, Vázquez E, Mangues R, Casanova I. A diphtheria toxin-based nanoparticle achieves specific cytotoxic effect on CXCR4(+) lymphoma cells without toxicity in immunocompromised and immunocompetent mice. BIOMEDICINE & PHARMACOTHERAPY. 2022; 150:112940. DOI:10.1016/j.biopha.2022.112940. PMID:35421785. IF:7,5 (Q1/1D). Document type: Article.
López H, Voltà E, Parladé E, Villaverde A, Vázquez E, Unzueta U. Insights on the emerging biotechnology of histidine-rich peptides. BIOTECHNOLOGY ADVANCES. 2022; 54:107817. DOI:10.1016/j.biotechadv.2021.107817. PMID:34418503. IF:16 (Q1/1D). Document type: Review.
Medina E, Cespedes MV, Gallardo A, Rioja-Blanco E, Pavon MA, Asensio L, Farre L, Alba-Castellón L, Unzueta U, Villaverde A, Vazquez E, Casanova I, Mangues R. Novel Endometrial Cancer Models Using Sensitive Metastasis Tracing for CXCR4-Targeted Therapy in Advanced Disease. Biomedicines. 2022; 10(7):1680. DOI:10.3390/biomedicines10071680. PMID:35884987. IF:4,7 (Q1/3D). Document type: Article.
Pallares V, Unzueta U, Falgas A, Avino A, Nunez Y, Garcia-Leon A, Sánchez-García L, Serna N, Gallardo A, Alba-Castellón L, Alamo P, Sierra J, Cedo L, Eritja R, Villaverde A, Vazquez E, Casanova I, Mangues R. A multivalent Ara-C-prodrug nanoconjugate achieves selective ablation of leukemic cells in an acute myeloid leukemia mouse model. BIOMATERIALS. 2022; 280:121258. DOI:10.1016/j.biomaterials.2021.121258. PMID:34847435. IF:14 (Q1/1D). Document type: Article.
Parlade E, Volta-Duran E, Cano-Garrido O, Sanchez JM, Unzueta U, López-Laguna H, Serna N, Cano M, Rodríguez-Mariscal M, Vazquez E, Villaverde A. An In Silico Methodology That Facilitates Decision Making in the Engineering of Nanoscale Protein Materials. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022; 23(9):4958. DOI:10.3390/ijms23094958. PMID:35563346. IF:5,6 (Q1/3D). Document type: Article.
Parra AM, Perez A, Gris I, Bonel GC, Carrasco LM, Mokhtari K, Garcia L, Lupianez JA, Rufino EE. Involvement of the PI3K/AKT Intracellular Signaling Pathway in the AntiCancer Activity of Hydroxytyrosol, a Polyphenol from Olea europaea, in Hematological Cells and Implication of HSP60 Levels in Its Anti-Inflammatory Activity. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022; 23(13):7053. DOI:10.3390/ijms23137053. PMID:35806065. IF:5,6 (Q1/3D). Document type: Article.
Rioja-Blanco E, Arroyo I, Alamo P, Casanova I, Gallardo A, Unzueta U, Serna N, Sánchez-García L, Quer M, Villaverde A, Vazquez E, Leon X, Alba-Castellón L, Mangues R. CXCR4-targeted nanotoxins induce GSDME-dependent pyroptosis in head and neck squamous cell carcinoma. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH. 2022; 41(1):49. DOI:10.1186/s13046-022-02267-8. PMID:35120582. IF:11,3 (Q1/1D). Document type: Article.
Rioja-Blanco E, Arroyo I, Álamo P, Casanova I, Gallardo A, Unzueta U, Serna N, Sánchez-García L, Quer M, Villaverde A, Vázquez E, Mangues R, Alba-Castellón L, León X. Self-assembling protein nanocarrier for selective delivery of cytotoxic polypeptides to CXCR4(+) head and neck squamous cell carcinoma tumors. Acta Pharmaceutica Sinica B. 2022; 12(5). DOI:10.1016/j.apsb.2021.09.030. PMID:35646535. IF:14,5 (Q1/1D). Document type: Article.
Rioja-Blanco E, Gallardo A, Arroyo I, Alamo P, Casanova I, Unzueta U, Serna N, Sánchez-García L, Quer M, Villaverde A, Vazquez E, Leon X, Alba-Castellón L, Mangues R. A Novel CXCR4-Targeted Diphtheria Toxin Nanoparticle Inhibits Invasion and Metastatic Dissemination in a Head and Neck Squamous Cell Carcinoma Mouse Model. Pharmaceutics. 2022; 14(4):887. DOI:10.3390/pharmaceutics14040887. PMID:35456719. IF:5,4 (Q1/2D). Document type: Article.
Sala R, Rioja-Blanco E, Serna N, Sánchez-García L, Alamo P, Alba-Castellón L, Casanova I, Lopez A, Unzueta U, Cespedes MV, Vazquez E, Villaverde A, Mangues R. GSDMD-dependent pyroptotic induction by a multivalent CXCR4-targeted nanotoxin blocks colorectal cancer metastases. DRUG DELIVERY. 2022; 29(1). DOI:10.1080/10717544.2022.2069302. PMID:35532120. IF:6 (Q1/2D). Document type: Article.
Sanchez JM, Carratala JV, Serna N, Unzueta U, Nolan V, Sánchez-Chardi A, Volta-Duran E, López-Laguna H, Ferrer-Miralles N, Villaverde A, Vazquez E. The Poly-Histidine Tag H6 Mediates Structural and Functional Properties of Disintegrating, Protein-Releasing Inclusion Bodies. Pharmaceutics. 2022; 14(3):602. DOI:10.3390/pharmaceutics14030602. PMID:35335976. IF:5,4 (Q1/2D). Document type: Article.
Serna N, Falgas A, Garcia-Leon A, Unzueta U, Nunez Y, Sánchez-Chardi A, Martínez-Torró C, Mangues R, Vazquez E, Casanova I, Villaverde A. Time-Prolonged Release of Tumor-Targeted Protein-MMAE Nanoconjugates from Implantable Hybrid Materials. 2022; 14(1):192. DOI:10.3390/pharmaceutics14010192. PMID:35057088. IF:5,4 (Q1/2D). Document type: Article.
Serna N, Pallares V, Unzueta U, Garcia-Leon A, Volta-Duran E, Sánchez-Chardi A, Parlade E, Rueda A, Casanova I, Falgas A, Alba-Castellón L, Sierra J, Villaverde A, Vazquez E, Mangues R. Engineering non-antibody human proteins as efficient scaffolds for selective, receptor-targeted drug delivery. JOURNAL OF CONTROLLED RELEASE. 2022; 343. DOI:10.1016/j.jconrel.2022.01.017. PMID:35051493. IF:10,8 (Q1/1D). Document type: Article.
Unzueta U, Sanchez JM, Garcia-Fruitos E, Seras J. Editorial: High added-value nanoparticles: Rethinking and recycling cell protein waste. Frontiers in Bioengineering and Biotechnology. 2022; 10:1018014. DOI:10.3389/fbioe.2022.1018014. PMID:36131721. IF:5,7 (Q1/3D). Document type: Editorial material.
Volta-Duran E, Sanchez JM, H, Parlade E, Sánchez-García L, Sánchez-Chardi A, de Marco A, Unzueta U, Vazquez E, Villaverde A. The spectrum of building block conformers sustains the biophysical properties of clinically-oriented self-assembling protein nanoparticles. Science China-Materials. 2022; 65(6). DOI:10.1007/s40843-021-1914-0. IF:8,1 (Q1/2D). Document type: Article.
Volta-Duran E, Sanchez JM, Parlade E, Serna N, Vazquez E, Unzueta U, Villaverde A. The Diphtheria Toxin Translocation Domain Impairs Receptor Selectivity in Cancer Cell-Targeted Protein Nanoparticles. 2022; 14(12):2644. DOI:10.3390/pharmaceutics14122644. PMID:36559138. IF:5,4 (Q1/2D). Document type: Article.

Publications 2021

Alamo P, Cedano J, Conchillo O, Cano O, Alba L, Serna N, Avino A, Carrasco LM, Sanchez A, Martinez C, Gallardo A, Cano M, Eritja R, Villaverde A, Mangues R, Vazquez E, Unzueta U. Rational engineering of a human GFP-like protein scaffold for humanized targeted nanomedicines. Acta Biomaterialia. 2021; 130. DOI:10.1016/j.actbio.2021.06.001. PMID:34116228. IF:10,633.
Alamo P, Parlade E, Lopez H, Volta E, Unzueta U, Vazquez E, Mangues R, Villaverde A. Ion-dependent slow protein release from in vivo disintegrating micro-granules. DRUG DELIVERY. 2021; 28(1). DOI:10.1080/10717544.2021.1998249. PMID:34747685. IF:6,819.
Cano O, Alamo P, Sanchez L, Falgas A, Sanchez A, Serna N, Parlade E, Unzueta U, Roldan M, Volta E, Casanova I, Villaverde A, Mangues R, Vazquez E. Biparatopic Protein Nanoparticles for the Precision Therapy of CXCR4(+) Cancers. Cancers. 2021; 13(12):2929. DOI:10.3390/cancers13122929. PMID:34208189. IF:6,575.
Falgas A, Pallares V, Unzueta U, Nunez Y, Sierra J, Gallardo A, Alba L, Mangues MA, Alamo P, Villaverde A, Vazquez E, Mangues R, Casanova I. Specific Cytotoxic Effect of an Auristatin Nanoconjugate Towards CXCR4(+) Diffuse Large B-Cell Lymphoma Cells. International Journal of Nanomedicine. 2021; 16DOI:10.2147/IJN.S289733. PMID:33716502. IF:7,033.
Lopez H, Parlade E, Alamo P, Sanchez JM, Volta E, Serna N, Sanchez L, Cano O, Sanchez A, Villaverde A, Mangues R, Unzueta U, Vazquez E. In Vitro Fabrication of Microscale Secretory Granules. ADVANCED FUNCTIONAL MATERIALS. 2021; 31(21):2100914. DOI:10.1002/adfm.202100914. IF:19,924.
Lopez H, Sanchez JM, Carratala JV, Rojas M, Sanchez L, Parlade E, Sanchez A, Volta E, Serna N, Cano O, Flores S, Ferrer N, Nolan V, de Marco A, Roher N, Unzueta U, Vazquez E, Villaverde A. Biofabrication of functional protein nanoparticles through simple His-tag engineering. ACS Sustainable Chemistry & Engineering. 2021; 9(36). DOI:10.1021/acssuschemeng.1c04256. PMID:34603855. IF:9,224.
Pallares V, Nunez Y, Sanchez L, Falgas A, Serna N, Unzueta U, Gallardo A, Alba L, Alamo P, Sierra J, Villaverde A, Vazquez E, Casanova I, Mangues R. Antineoplastic effect of a diphtheria toxin-based nanoparticle targeting acute myeloid leukemia cells overexpressing CXCR4. JOURNAL OF CONTROLLED RELEASE. 2021; 335. DOI:10.1016/j.jconrel.2021.05.014. PMID:34004204. IF:11,467.
Sanchez JM, Lopez H, Serna N, Unzueta U, Clop PD, Villaverde A, Vazquez E. Engineering the Performance of Artificial Inclusion Bodies Built of Catalytic beta-Galactosidase. ACS Sustainable Chemistry & Engineering. 2021; 9(6). DOI:10.1021/acssuschemeng.0c08345. IF:9,224.
Sanchez L, Volta E, Parlade E, Mazzega E, Sanchez A, Serna N, Lopez H, Mitstorfer M, Unzueta U, Vazquez E, Villaverde A, de Marco A. Self-Assembled Nanobodies as Selectively Targeted, Nanostructured, and Multivalent Materials. ACS Applied Materials & Interfaces. 2021; 13(25). DOI:10.1021/acsami.1c08092. PMID:34129336. IF:10,383.
Serna N, Carratala JV, Conchillo O, Martinez C, Unzueta U, Mangues R, Ferrer N, Daura X, Vazquez E, Villaverde A. Antibacterial Activity of T22, a Specific Peptidic Ligand of the Tumoral Marker CXCR4. Pharmaceutics. 2021; 13(11):1922. DOI:10.3390/pharmaceutics13111922. PMID:34834337. IF:6,525.
Volta E, Serna N, Sanchez L, Avino A, Sanchez JM, Lopez H, Cano O, Casanova I, Mangues R, Eritja R, Vazquez E, Villaverde A, Unzueta U. Design and engineering of tumor-targeted, dual-acting cytotoxic nanoparticles. Acta Biomaterialia. 2021; 119. DOI:10.1016/j.actbio.2020.11.018. PMID:33189955. IF:10,633.

ONCOHEMATOLOGIC DISEASES

Oncogenesis and Antitumour Drugs

Main Lines of Research

Challenges

Group coordination

Members

Publications 2023

Publications 2022

Publications 2021

2017 Scientific Report -Oncogenesis and Antitumour

‘Agència de Gestió d’Ajuts Universitaris i de Recerca’ (AGAUR) funding:

U18. Nanotoxicology Unit (NANBIOSIS):

Main Lines of Research

Challenges

Group coordination

Members

Publications 2023

Publications 2022

Publications 2021

Institut de Recerca de l’Hospital de la Santa Creu i Sant Pau